qTOWER³

qTOWER3 – Get in touch with high-class qPCR

Vision, Amazement, Simplicity: the new qTOWER³ real-time PCR thermal cycler from Analytik Jena.

The patented fiber optic shuttle system with unique light source powered by 4 high-performance LED’s guarantees ideal excitation and detection of known fluorescent dyes up to the dark red. In addition, the system’s highly sensitive detection module can be equipped with up to 6 different filter modules. Filter modules are upgradeable, allowing the system to benefit from future Analytik Jena developments. And, enjoy peace of mind knowing that the qTOWER³’s optical components are backed by an industry-leading 10-year warranty.

Silver block technology is at the heart of the qTOWER³, offering outstanding control accuracy of only ±0.1 °C over the entire 96 well block. Optionally, the linear gradient function is the optimal tool to easily adjust the instrument to new assays. The qTOWER³ is available either as stand-alone device with integrated 10” tablet or as PC-based system. The software comes with a broad spectrum of optimized analysis algorithms including absolute and relative quantification, ddCt method, PCR efficiency, allelic discrimination, endpoint detection as well as melt point and protein analysis.

  • Description
  • Features
  • Technical Data

Description

Patented Fiber Optic Shuttle System
In the field of quantitative real-time PCR a wide variety of fluorescent dyes with different characteristics are applicable. In order to optimally excite each of these dyes over a wide spectral range, a novel light source with four long-life LEDs in red, green, blue and white (RGBW) is used in qTOWER³. The RGBW-LED ensures best possible quantum yield of each dye in real-time PCR experiments. Highly sophisticated multiplex applications with up to 6 fluorescent-labeled probes from the blue to the near-infrared range are no challenge anymore. Fiber Optic System (FOS):The integrated shuttle system with 8 optical fibers ensures an extremely fast read-out of the entire 96 well plate in only 6 seconds – irrespective of the number of dyes to be measured. In addition, all components of the high-performance optics come with a 10-year long-term guarantee.

Maximum flexibility
Depending on the application qTOWER³’s Filter Module System can be freely configured and upgraded, to both intercalating and protein-binding dyes, as well as to hydrolysis (e.g. Taqman®) and hybridization (FRET) probes. The system is future-proof, being easily retrofitted on-site with additional Color, FRET or Protein Modules, and can thus be adapted to other fields of application at any time. All filter modules contain an optimized combination of excitation and emission filters which, in combination with the light source,enable the ideal detection of a multitude of commercially available fluorescent dyes.

Innovative Silver Block Technology
Basis for a reliable performance of quantitative real-time PCR in qTOWER³ is the sample block. To ensure best possible results and outstanding energy transfer into the sample, the thermal block is made of gold-coated silver serving much better physical properties than aluminum. As a result, the qTOWER³ is characterized by excellent temperature control accuracy of ±0.1 °C (over 96 wells) and first-class heating rates of up to 8 °C/sec. This combination makes the overall system the ultimate for any real-time PCR application. To guarantee the highest specificities for different assays, the device is available with a gradient function of up to 40 °C span over 12 columns. Rather than defining upper and lower temperatures as limits, the Linear Gradient Tool (LGT) allows to define simply the temperature increments as integer around the calculated annealing temperature of primers in a linear fashion thus making operation of the qTOWER³ simple and intuitive.

To avoid condensation and the resulting loss of samples, the system is equipped with a motorized heated lid. This can be variably set up to 110 °C and guarantees, regardless of the consumables used an optimal contact pressure on the sample vessels during the entire real-time PCR run.

Intuitive Operation and Automatic Evaluation

The control and evaluation software qPCRsoft follows the concept that it should be flexible and user friendly. The logical arrangement of all tools, the intuitive handling and, last but not least, the parameter-oriented memory and programming design make the use of the software simple and clear. During an current run, the data from previous experiments can be evaluated in parallel without complication.

In order to facilitate operation, a variety of steps, such as threshold calculation for determination of Ct-values or possible standard curves and PCR efficiencies are done automatically. Further evaluations such as absolute or relative quantifications as well as the ddCt-method (with or without relation to the PCR efficiency) can likewise be carried out automatically. qPCRsoft also includes algorithms for probe-based assays, allelic discrimination for e.g. determination of point mutations and the use of a positive/ negative analysis by endpoint analysis of the amplification plots.

Get in touch with qTOWER³
qTOWER³ can be optionally expanded with the aid of a through-and-through innovative operation concept. The qTOWER³ touch is a modern stand-alone system which renders the need of an external control system unnecessary. The integrated 10“ tablet leaves nothing to be desired with regard to experiment planning and implementation. The touch operation provides the user with a simple, intuitive menu navigation and both cycler programming as well as online monitoring and final Ct-value determination. The data files are compatible at all times with the comprehensive PC software and easily allow following evaluations. Regardless of whether it is qTOWER³ or qTOWER³ touch – the real-time PCR thermal cyclers from Analytik Jena are the ideal and reliable tool for everyday lab work.

Regardless of whether it is qTOWER³ or qTOWER³ touch – the real-time PCR thermal cyclers from Analytik Jena are the ideal and reliable tool for everyday lab work.

qTOWER3 - Real-Time PCR Thermal Cycler

  • Hands-on: Stand-alone operation via integrated tablet control (10“) and/or enhanced PC control
  • Transparent: No costs for license or updates (license-free)
  • Universal: Covering a broad range of analysis tools from a simple overview of Ct values to ddCT method and multi plate analysis
  • Multilingual: Available in multiple different languages
  • Convenient: 12 available Color, FRET and Protein Modules can be installed or exchanged within a few minutes
  • Future-proof: Adaptable to new applications at any time by adding filter modules
  • Robust: Patented high performance optical system with an outstanding long-term warranty of 10 years!
  • Efficient: Short scan times of 6 seconds for the entire plate, irrespective of the number of dyes detected
  • Innovative: Novel light source with 4 robust LEDs (RGBW) – no preheating time, long life-time
  • Brillant: Ideal illumination and detection of all 96 wells individually without edge effect due to fiber optic shuttle
  • Fast: Heating rates of 8 °C/sec and cooling rates of 6 °C/sec
  • Unrivaled: Ideal temperature homogeneity and unmatched control accuracy (± 0.1 °C)
  • Precise: The temperature difference from lane to lane is the same; simple programming of even gradient temperatures is possible with our unique Linear Gradient Tool (LGT)
Thermal block
Sample block Silver sample block with gold-coating
Block capacity 96 Well á 0.2 ml for 96 x 0.2 ml
Sample size 10 – 80 μl
Heating Up to 8 °C/sec (max.)
Cooling Up to 6 °C/sec (max.)
Adjustable temperature range 4 °C to 99 °C
Temperature uniformity 55 °C ± 0.15 °C after 15 sec
Temperature control accuracy ± 0.1 °C
Gradient Linear Gradient Tool
Max./Min. Gradient 40 °C / 0.1 °C
Heated lid
Lid temperature 30 °C to 110 °C
Contact pressure 30 kg, automated
qPCR application
Sensitivity Detects 1 copy of target sequence in human genomic DNA
Dynamic range 10 orders of magnitude
Optics
Measuring principle Fiber optic shuttle system with 8-fold scanner and color modules
for excitation and emission filters
Light source 4 longlife, high-power LED’s (RGBW)
Detector High sensitive PMT
(Photo Multiplier Tube)
Color modules
  • 12 Color-, FRET- and Protein Modules
  • 6 positions inside device
Read out time 6 sec for 96 wells independent of the number of dyes
Control
Control PC or stand-alone (optional)
Display Only qTOWER³ touch: 10“ tablet, colored, touch, WIN 8.1
Time inc/dec ±1 to 240 sec/cycle
Temperature inc/dec ±0.1 to 20 °C/cycle
Additional technical data
Weight Approx. 30 kg
Dimension
(W x H x L)
275 mm x 585 mm x 275 mm
Interface
  • ƒƒPC connection: USB, RS232,
  • Tablet: USB for data transfer, barcode reader
Noise emission 45 db
Warranty
  • 2 warranty on device system
  • 10 years long-term warranty of high performance optics
Parameters: color modules
Name Excitation Emission Dyes* (examples)
Color module 1 470 nm 520 nm FAMTM, Sybr®Green, Alexa488®
Color module 2 515 nm 545 nm JOETM, HEXTM, VIC®, YakimaYellowTM
Color module 3 535 nm 580 nm TAMRATM, DFOTM, Alexa546®, NEDTM
Color module 4 565 nm 605 nm ROXTM, TexasRed®, Cy3.5®
Color module 5 630 nm 670 nm Cy5®, Alexa633®, Quasar670TM
Color module 6 660 nm 705 nm Cy5.5®, LightCycler Red®
FRET module 1 470 nm 580 nm FAMTM (donor) / TAMRATM(acceptor)
FRET module 2 470 nm 670 nm FAMTM (donor) / Cy5®(acceptor)
FRET module 3 470 nm 705 nm FAMTM (donor) / Cy5.5®(acceptor)
FRET module 4 515 nm 670 nm JOETM (donor) / Cy5®(acceptor)
FRET module 5 470 nm 605 nm FAMTM (Donor)/ROXTM (acceptor)
Color modul Protein 1 490 nm 580 nm SYPRO® Orange